SUMOylation of HNF4a regulates protein stability and hepatocyte function
نویسندگان
چکیده
Wenli Zhou*, Zara Hannoun*, Ellis Jaffray, Claire N. Medine, James R. Black, Sebastian Greenhough, Liang Zhu, James A. Ross, Stuart Forbes, Ian Wilmut, John P. Iredale, Ronald T. Hay and David C. Hay Medical Research Council Centre for Regenerative Medicine, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UK Changzheng Hospital, Second Military Medical University, Shanghai 200003, China Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK Medical Research Council Centre for Inflammation Research, University of Edinburgh, QMRI, 47 Little France Crescent, Edinburgh EH16 4TJ, UK
منابع مشابه
SUMOylation of HNF4α regulates protein stability and hepatocyte function
The coordination of signalling pathways within the cell is vital for normal human development and post-natal tissue homeostasis. Gene expression and function is therefore tightly controlled at a number of levels. We investigated the role that post-translational modifications play during human hepatocyte differentiation. In particular, we examined the role of the small ubiquitin-like modifier (S...
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We have previously shown that the transcription factor HNF4A is required for the formation of hepatic progenitor cells from endoderm that has been derived from human induced pluripotent stem cells. We reasoned that we could uncover regulatory pathways with novel roles in hepatocyte differentiation by identifying cellular processes that regulate HNF4A. We, therefore, performed a screen of 1120 s...
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The functions of the TAF subunits of mammalian TFIID in physiological processes remain poorly characterised. In this study, we describe a novel function of TAFs in directing genomic occupancy of a transcriptional activator. Using liver-specific inactivation in mice, we show that the TAF4 subunit of TFIID is required for post-natal hepatocyte maturation. TAF4 promotes pre-initiation complex (PIC...
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We have previously shown that the transcription factor HNF4A is required for the formation of hepatic progenitor cells from endoderm that has been derived from human induced pluripotent stem cells (iPSCs). We reasoned that we could uncover regulatory pathways with new roles in hepatocyte differentiation by identifying cellular processes that regulate HNF4A. We therefore performed a screen of 11...
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The forkhead box protein A2 (FOXA2) is an important regulator of glucose and lipid metabolism and organismal energy balance. Little is known about how FOXA2 protein expression and activity are regulated by post-translational modifications. We have identified that FOXA2 is post-translationally modified by covalent attachment of a small ubiquitin related modifier-1 (SUMO-1) and mapped the sumoyla...
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